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一种新型体外胆管灌注系统模型建立的可行性研究

发布日期:2014-10-22 12:21:41 文章来源: 作者次数:1566

    【摘要】
        目的 建立一种新型的体外胆管灌注系统模型,探讨各种胆管内试验在这种模型里进行同质对比研究的可行性。
        方法 设计一种10个样品试验区的体外胆管灌注系统模型,将生理盐水、甘油和猪胆汁分别放入这种模型里,将第一个样品区的流量设定在8滴/min,在0.5、1.0、1.5、2.0、12.0和24.0 h末收集并测量这种模型的10个样品区流出的液体量,评估这些液体量在每一个时间点是否相等。10种含依地酸二钠不同含量的、相同规格的缓释药棒分别放入这种模型的试验区,在注入这些液体后,在24.0 h末收集并分析这10个试验区的依地酸二钠浓度,评估它们是否相等。同时,评估这3种液体对同一种药物缓释棒是否相等。
        结果 在相同时间点,流经10个样品试验区的生理盐水、甘油和猪胆汁是相等的,差异无统计学意义(P>0.01);但是对于同一种液体,依地酸二钠浓度在24.0 h末不同(P<0.01);对于同一种药物缓释棒,不同的液体也有差异(P<0.01)。
    结论 这种模型能够获得相同流量和实验环境,当做不同的实验时能够在不同的试验区获得不同结果,当实验环境发生改变时同一种样品的结果也会发生相应的改变,可以模拟胆管环境进行体外试验研究。
    【关键词】胆管;体外研究;灌注系统;实验模型


    【Abstract】
    Objective   To establish a new model of biliary perfusion in vitro, and to explore the feasibility of comparative biliary studies with the model.
    Methods   A set of ten-test-area model of biliary perfusion was designed in vitro. Saline, glycerol and pig bile were put into this model. The outflow of the first tube was set at 8 drops/min, and these liquids were collected through the ten sample test areas and measured at 0.5 hour, 1.0 hour, 1.5 hours, 2.0 hours, 12.0 hours and 24.0 hours. Equality of liquid amount was evaluated from each sample test area. Rods containing delayed release EDTA of ten different levels were placed in the test areas. The liquid was collected and EDTA concentrations were evaluated from the ten tubes at 24.0 hours. EDTA concentrations of the same rod in the three liquids were compared.
    Results   At the same time point, the amounts of saline, glycerol, pig bile flowing through the ten sample test areas showed no significant difference (P>0.01); but EDTA concentrations of the same liquid at 24.0 hours were significantly different (P<0.01); and EDTA concentrations of the same rod in the three liquids were also obviously different (P<0.01).
    Conclusion   The same liquid flow rates and the same experimental environment can be achieved. Different test results can be obtained from different test areas in different experiments. The test results of the same kind of sample change with the changes of experimental conditions, which simulates biliary duct.
    【Key words】Bile ducts;In vitro;Perfusion system;Experimental model                        

    (作者:张伟星 李斌 李百文 李雷 宛新建)
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